Cryopreservation of periwinkle,Catharanthus roseus cells cultured in vitro |
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Authors: | K. K. Kartha N. L. Leung P. Gaudet-LaPrairie F. Constabel |
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Affiliation: | (1) Prairie Regional Laboratory, National Research Council of Canada, S7N 0W9 Saskatoon, Sask., Canada |
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Abstract: | A procedure for prolonged cryogenic storage of periwinkle cell cultures is described. Cells derived from periwinkle, Catharanthus roseus (L.) G. Don, and subcultured as suspension in 1-B5C nutrient medium have been frozen, stored in liquid nitrogen (–196°C) for 11 weeks, thawed and recultured. Maximal survival was achieved when 3–4 day-old cells precultured for 24 h in nutrient medium with 5% DMSO were frozen at slow cooling rates of 0.5 or 1°C/min prior to storage in liquid nitrogen. The only loss in viability of cells occurred subsequent to treatment with DMSO. Abbreviations: DMSO, dimethylsulfoxide; 2,4-D, 2,4-dichlorophenoxyacetic acid; TTC, triphenyltetrazolium chloride.NRCC No. 20082 |
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Keywords: | Catharanthus roseus periwinkle cell cultures DMSO cryopreservation cryogenic storage |
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