白芍传统品种原植物遗传多样性及亲缘关系分析 |
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引用本文: | 陈丙銮,杭悦宇,周义锋,郭可跃,黄春洪,李正.白芍传统品种原植物遗传多样性及亲缘关系分析[J].植物资源与环境学报,2003,12(2):17-21. |
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作者姓名: | 陈丙銮 杭悦宇 周义锋 郭可跃 黄春洪 李正 |
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作者单位: | 江苏省,中国科学院,植物研究所(南京中山植物园),江苏,南京,210014 |
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基金项目: | 江苏省自然科学基金资助项目(BK2000032),连云港康缘制药有限责任公司横向合作项目 |
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摘 要: | 为探讨白芍原植物各居群间的遗传多样性及亲缘关系,本实验应用RAPD技术,使用40条随机引物对10个白芍原植物居群的叶片总DNA进行PCR扩增。结果表明:白芍10个原植物居群间具有较丰富的遗传多样性,多态率达63.以%。由结合线L=0.7975可将10个居群划分为6组,原植物为芍药(Paeonia lactiflora Pall.)的6个居群中有4个居群集中在第3组,显示了种内遗传关系的相似性,其中川白芍(芍药)花色有变,同时雄蕊有部分辨化的现象,与野生种(韩城芍药)有较远的遗传关系;山东荷泽居群雄蕊全部瓣化,种质可能来自于观赏芍药突变体,在遗传关系上与芍药的其他居群有一定的距离。原植物为毛果芍药P.lactiflora var.trichocarpa (Bunge) Stern]的4个居群中,杭白芍粉红花居群和白花居群独立成一组,与形态分类的结果一致。芍药及其变种毛果芍药的遗传关系非常近,但是有交叉。因此形态分类和遗传关系应综合考虑,作为种下分类单位的确定也应慎重。
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关 键 词: | 白芍 居群 遗传多样性 亲缘关系 RAPD技术 分类 原植物 中国传统中药 |
文章编号: | 1004-0978(2003)02-0017-05 |
修稿时间: | 2003年2月20日 |
Genetic diversity and relationship of different populations of original plants of Radix Paeonia Alba |
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Abstract: | RAPD is used to study the genetic diversity and relationship of plant populations of Radix Paeonia Alba, in which 40 random primers are used. Ther e are rich genetic diversities among 10 different populations (the polymorphic ratio is 63.67%). Based on the combining line (L=0.797 5), ten popul ations can be classified into 6 groups. The 4 populations in 6 Paeonia lactifl ora Pall. populations are intensively clustered in section 3, which reveals a high genetic ident ify. The population of Chuanbaishao ( P. lactiflora ) in Zhongjiang, Sichuan Province is outgrouped by different flower color and partial ly petaloid stamens, it shows a long genetic distance between the cultivar and w ild in sect. 3. The population of P. lactiflora from Heze, Shandong Province , as the descendan t of a probable mutation of the decorative cultivars, is characterized with comp letely petaloid stamens and separated from others in heredity. Among the 4 popul ations of P. lactiflora var. trichocarpa (Bunge) Stern, the white and pi nk flower populations of Hangbaishao from Pan'an, Zhejiang Province are cluster ed into a separate section, which is consistent with the result based on morphol ogical classification. P. lactiflora has a close relationship with its var iety P. lactiflora var. trichocarpa . We should compre hensively deliberate on the classification of units infraspecific and tak e a consideration based on morphology and genetic distance. |
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Keywords: | Radix Paeonia Alba population genetic diversi ty RAPD |
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