Quantitative assessment of the tetracycline resistance gene pool in cheese samples by real-time TaqMan PCR |
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| Authors: | Manuzon Michele Y Hanna Scott E Luo Hongliang Yu Zhongtang Harper W James Wang Hua H |
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| Affiliation: | Department of Food Science and Technology, The Ohio State University, Parker FST Building, 2015 Fyffe Ct., Columbus, OH 43210-1007, USA. |
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| Abstract: | A TaqMan real-time PCR assay was developed to quantify the tetS gene pool present in retail cheeses. This protocol offers a rapid, specific, sensitive, and culture-independent method for assessing antibiotic resistance genes in food samples rich in fats and proteins. |
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