Simultaneous uptake of galactose and glucose by Azotobacter vinelandii. |
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Authors: | T Y Wong C A Murdock S P Concannon T D Lockey |
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Institution: | Biology Department, Memphis State University, TN 38152. |
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Abstract: | Azotobacter vinelandii growing on galactosides induced two distinct permeases for glucose and galactose. The apparent Vmax and Km of the galactose permease were 16 nmol galactose/min per 10(10) cells and 0.5 mM, respectively. The apparent Vmax and Km of the glucose permease were 7.8 nmol glucose/min per 10(10) cells and 0.04 mM, respectively. Excess glucose had no effect on the galactose uptake. However, excess galactose inhibited glucose transport. The galactosides-induced glucose permease also exhibited different uptake kinetics from that induced by glucose. |
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