Statistical study of rapid mechanodependent cell movements in deformed explants of african clawed frog <Emphasis Type="Italic">Xenopus laevis</Emphasis> embryonic tissues |
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Authors: | T G Troshina N S Glagoleva L V Belousov |
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Institution: | (1) Department of Embryology, Faculty of Biology, Moscow State University, Moscow, 119899, Russia; |
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Abstract: | Computer analysis of artificially deformed (stretched or compressed) double explants (sandwiches) of the blastocoel roof (BRs)
and suprablastoporal region (SBRs) of African clawed frog Xenopus laevis early gastrula has been performed using frames of time-lapse microfilming. During the first 14 min after cutting off, the
velocities and displacement angles of several hundreds of cells relative to one another, as well as to fixed points and the
extension axis, were measured in the control and deformed samples. It has been found that the deformation of samples leads
to a rapid reorientation of large cell masses and increase in the velocities of movements along the extension axes or perpendicularly
to the compression axes. In addition, an increase in the velocities of mutual cell displacements in the stretched BRs and
cell convergence to the extension axes have been observed. Comparison of different angular sectors demonstrates a statistically
significant positive correlation between the mean velocities of cell movements and the number of cells moving within an individual
sector. This suggests cooperativity of mechanodependent cell movements. In general, these results demonstrate an important
role of mechanical factors in regulation of collective cell movements. |
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Keywords: | |
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