Kinetic formulations for the reduction of ketomalonate by lactate dehydrogenase |
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| Authors: | I Haro J Li Gelpí A Mazo A Cortés |
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| Institution: | Departament de Bioquimica i Fisiologia, Facultat de Química, Universitat de Barcelona, Spain. |
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| Abstract: | Initial rate kinetic studies of lactate dehydrogenase with ketomalonate and NADH as substrates suggest that this enzymatic system is adapted to a rapid equilibrium ordered bi-bi ternary complex mechanism. The application of the reaction product inhibition method reveals the existence of the enzyme-NADH-hydroxymalonate and enzyme-NAD(+)-ketomalonate abortive complexes. This kinetic behaviour is confirmed by the differential inhibition induced by several alternate products on the pyruvate-lactate dehydrogenase-NADH and ketomalonate-lactate dehydrogenase-NADH systems. |
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