Cloning, sequencing and expression of a recA gene from Amycolatopsis mediterranei |
| |
| Authors: | Jin Gao Weihong Jiang Guoping Zhao Juishen Chiao |
| |
| Affiliation: | (1) Laboratory of Molecular Regulation for Microbial Secondary Metabolism, Shanghai Institute of Plant Physiology, Chinese Academy of Sciences, 300 Fenglin Road, Shanghai, 200032, China;(2) 150 Morgan Hall#3104, University of California at Berkeley, Berkeley, CA 94720, USA;(3) Shanghai Research Center of Biotechnology, Chinese Academy of Sciences, Shanghai, 200233, China |
| |
| Abstract: | The 3.5 kb nucleotide fragment, including the recA gene and its downstream recX-like gene, has been isolated from a genomic library by dot-blot hybridization with the Mycobacterium smegmatis recA gene. The recA gene, consisting of 1047 base pairs (bp), encodes a polypeptide of 348 amino acids while the recX-like gene, consisting of 450 bp, encodes a shorter polypeptide of 149 amino acids. Both the deduced amino acid sequences of recA and recX resemble those of the recA and recX genes from other bacteria. The cloned Amycolatopsis mediterranei U32 recA gene conferred partial resistance to ethyl methane sulfonate when expressed in E. coli with the lacZ promoter. |
| |
| Keywords: | Amycolatopsis mediterranei expression recA gene recX gene |
| 本文献已被 SpringerLink 等数据库收录！ |
|
