In vitro plant regeneration of Vismia guianensis through organogenesis |
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Authors: | Monacelli Barbara Pasqua Gabriella Cuteri Angelina Vitali Alberto |
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Institution: | (1) Dipartimento di Biologia Vegetale, Università ‘La Sapienza’, P.le Aldo Moro 5, 00185 Roma, Italy;(2) Centro Chimica dei Recettori, Istituto di Chimica, Università Cattolica S. Cuore, Roma, Italy |
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Abstract: | A protocol for in vitro plant regeneration through organogenesis was established for Vismia guianensis(Hypericaceae), a species that produces an anti-cancer compound. The highest mean number of shoots per gram of callus (57.33)
was obtained on Murashige and Skoog medium supplemented with 4.44 μM 6-benzyl-aminopurine, 5.70 μM indole-3-acetic acid and
12.88 μM gibberellic acid. Rooting was favoured by the addition of 10 μM indole-3-butyric acid, and by sucrose concentrations
higher than 1%.
This revised version was published online in June 2006 with corrections to the Cover Date. |
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Keywords: | plant regeneration tissue culture Vismia guianensis |
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