Nucleotide sequence of the genes coding for alpha, beta and gamma subunits of the proton-translocating ATPase of Escherichia coli |
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Authors: | H Kanazawa T Kayano K Mabuchi M Futai |
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Institution: | Department of Microbiology, Faculty of Pharmaceutical Sciences Okayama University, Okayama 700, Japan |
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Abstract: | A nucleotide sequence of 2328 base pairs comprising a portion of the gene cluster for the proton-translocating ATPase of was determined. The sequence covers most of the gene for α subunit, the entire gene for γ subunit and the amino terminal portion of the gene for β subunit, along with the flanking regions of these genes. The amino acid sequences of these subunits deduced from the DNA sequences indicate that the α and γ subunits have 513 and 287 amino acid residues, respectively. A possible secondary structure for each subunit was estimated from the inferred primary structure. The intercistronic regions between the genes for α and γ and between γ and β are 49 and 26 base pairs, respectively. The significance of codon usage in these genes is discussed in correlation with their expression. |
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Keywords: | periphral and integral membrane portions of proton-translocating ATPase periphral and integral membrane portions of proton-translocating ATPase DCCD N N′-dicyclohexylcarbodiimide DCCD-bp |
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