| Abstract: | 13 non-linked chromosomal mutations derepress the negative genetic control of copy number of K2 yeast killer plasmids and lead to 1.5-2-fold elevating of copy number of that type plasmids -L2A and M2 virus-like dsRNA. The content of both plasmids is increased 3-5-fold in cells with chromosomal ski5 mutation, as compared to the strains of wild type. Expression of ski5 allele is recessive. The dose effect of this allele is observed on haploid and diploid levels. Dominant ochre nonsense suppressors suppress the action of ski5 and the ski6 allele epistates that of ski5. |
