| 转天麻抗真菌蛋白GAFP基因烟草的获得及离体抑菌活性检测 |
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| 引用本文： | 陈英,王义琴,诸葛强,施季森,黄敏仁,王明庥. 转天麻抗真菌蛋白GAFP基因烟草的获得及离体抑菌活性检测[J]. 植物资源与环境学报, 2002, 11(2): 1-5 |
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| 作者姓名： | 陈英 王义琴 诸葛强 施季森 黄敏仁 王明庥 |
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| 作者单位： | 1. 南京林业大学林木遗传与基因工程重点实验室，江苏 南京，210037
2. 中国科学院遗传与发育生物研究所，北京，100101 |
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| 基金项目： | 国家自然科学基金农业倾斜项目(3017074) |
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| 摘 要： | 通过根癌农杆菌(Agrobacteriumtumefaciens)介导,采用叶盘法将天麻(GastrodiaelataBl.)抗真菌蛋白基因GAFP转入烟草(NicotianatabacumL.),PCR和Southern blotting分析证明已将GAFP基因整合进烟草植株。体外抑菌实验表明GAFP转基因的烟草植株对真菌瑞氏木霉(Trichodermareesei)表现出一定的抑菌活性。
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| 关 键 词： | 根癌农杆菌介导 天麻抗真菌蛋白 转基因烟草 离体抑菌活性 |
| 文章编号： | 1004-0978(2002)02-0001-05 |
| 修稿时间： | 2002-01-31 |
Acquirement of tabacco with transformation genes of GAFP (Gastrodia Antifungal Protein) and evalution of antifungal activity in vitro |
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| CHEN Ying {},WANG Yi|qin {},ZHUGE Qiang {},SHI Ji|sen {},HUANG Min|ren {},WANG Ming|xiu {}. Acquirement of tabacco with transformation genes of GAFP (Gastrodia Antifungal Protein) and evalution of antifungal activity in vitro[J]. Journal of Plant Resources and Environment, 2002, 11(2): 1-5 |
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| Authors: | CHEN Ying {} WANG Yi|qin {} ZHUGE Qiang {} SHI Ji|sen {} HUANG Min|ren {} WANG Ming|xiu {} |
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| Affiliation: | CHEN Ying {1},WANG Yi|qin {2},ZHUGE Qiang {1},SHI Ji|sen {1},HUANG Min|ren {1},WANG Ming|xiu {1} |
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| Abstract: | Mediated by %Agrobacterium tumefaciens%, the gene of Gastrodia Antifungal Protein (GAFP) was transferred into tobacco (%Nicotiana tabacum %L.) using the leaf|disk method. Results of PCR and Southern|blotting proved that GAFP gene was integrated into the tobacco genome. Extracts of transgenic lines showed resistance to the growth of %Trichoderma reesei in vitro, %which suggests that the GAFP gene was expressed efficiently in transgenic tobacco and denoted antifungal activity. |
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| Keywords: | Agrobacterium tumefaciens mediated transformation Gastrodia Antifungal Protein transgenic tobacco antifungal activity %in vitro% |
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