RNAi screening for fat regulatory genes with SRS microscopy |
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| Authors: | Wang Meng C Min Wei Freudiger Christian W Ruvkun Gary Xie X Sunney |
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| Affiliation: | Department of Molecular and Human Genetics and Huffington Center on Aging, Baylor College of Medicine, Houston, Texas, USA. |
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| Abstract: | Identification of genes regulating fat accumulation is important for basic and medical research; genetic screening for those genes in Caenorhabditis elegans, a widely used model organism, requires in vivo quantification of lipids. We demonstrated RNA interference screening based on quantitative imaging of lipids with label-free stimulated Raman scattering (SRS) microscopy, which overcomes major limitations of coherent anti-Stokes Raman scattering microscopy. Our screening yielded eight new genetic regulators of fat storage. |
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