Rapid isolation and sequence analysis of the beta-tubulin gene from <Emphasis Type="Italic">Porphyra yezoensis</Emphasis> (Rhodophyta) |
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Authors: | Qianhong?Gong Feng?Han Jixun?Dai Hongquan?Liu Huashi?Guan Email author" target="_blank">Wengong?YuEmail author |
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Institution: | (1) Marine Drug and Food Institute, Ocean University of China, 5 Yushan Road, Qingdao, 266003, People’s Republic of China |
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Abstract: | Beta-tubulin, one of the cytoskeletal proteins, has been highly conserved throughout the evolution of eukaryotes. Degenerate
PCR and inverse PCR (iPCR) were used to isolate the full-length beta-tubulin gene and its 5′ and 3′-flanking regions (2799bp)
from the marine red alga Porphyra yezoensis. This gene, designated as TubB1, is devoid of introns. The canonical cis-acting elements such as TATA box, CAAT box and polyA signal AAUAAA are not found
in flanking sequences, but another putative polyA signal CAYTG is found downstream of the stop codon. Comparison of the deduced
458 amino acid sequences shows higher similarity to the Protoflorideophycidae Cyanidioschyzon merolae (82%) than to the red alga Chondrus crispus (79%). Codon bias indicates strong expression of TubB1. Phylogenetic analysis suggests that the beta-tubulin of P. yezoensis and C. merola go together with fungi and not with green plants. These nucleotide sequence data have been deposited in the DDB/EMBL/Genbank
databases under the accession number AY221630. |
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Keywords: | beta-tubulin intronless inverse PCR Porphyra yezoensis Rhodophyta sequence analysis |
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