| 芍药组织培养中污染现象的克服 |
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| 引用本文： | 王吉凤,李青,包欣. 芍药组织培养中污染现象的克服[J]. 植物研究, 2012, 32(1): 84-90 |
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| 作者姓名： | 王吉凤 李青 包欣 |
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| 作者单位： | 北京林业大学园林学院国家花卉工程技术中心 |
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| 基金项目： | 十一五国家科技支撑计划项目(2006BAD07B01) |
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| 摘 要： | 针对芍药在组织培养过程中外植体污染难于控制的问题进行了研究。试验分别于不同的季节取材后对外植体进行不同的表面消毒处理。结果表明：以芍药带芽茎段、顶芽、侧芽为外植体，采用0.1%~0.2% HgCl2消毒8 min，结合母株黄化处理、4℃消毒液+搅拌的表面消毒处理，可降低外植体初期培养污染率；在培养基中添加抗菌素，可明显控制内生菌的污染。
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| 关 键 词： | 芍药；外植体；组织培养；污染；抗菌素 |
Contamination Control during Tissue Culture of Peony |
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| WANG Ji-Feng LI Qing,BAO Xin. Contamination Control during Tissue Culture of Peony[J]. Bulletin of Botanical Research, 2012, 32(1): 84-90 |
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| Authors: | WANG Ji-Feng LI Qing BAO Xin |
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| Affiliation: | (School of Landscape Architecture,Beijing Forestry University,National Engineering Research Center for Flowers,Beijing 100083) |
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| Abstract: | To solve the problem of explant contamination during peony tissue culture,the explants of different seasons were studied.The results showed that soaking explants in 0.1%-0.2% HgCl2 for 8min,etiolation treatment,low temperature and electronic vibration are effective for sterilization;Antibiotics added into the media can inhibit contamination caused by endophytes. |
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| Keywords: | peony explant tissue culture contamination antibiotics |
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