| 橡胶草HMGR基因的克隆及表达分析 |
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| 引用本文： | 王启超,刘实忠,校现周. 橡胶草HMGR基因的克隆及表达分析[J]. 植物研究, 2012, 32(1): 61-68 |
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| 作者姓名： | 王启超 刘实忠 校现周 |
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| 作者单位： | 海南大学园艺园林学院;中国热带农业科学院橡胶研究所 |
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| 基金项目： | 中国热带农业科学院橡胶研究所基本业务专项(No.xjszx_06) |
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| 摘 要： | 通过比较9种植物的9条甲羟戊酸途径关键酶3-羟基-3甲基戊二酸单酰辅酶A还原酶（HMGR）氨基酸同源区域，设计简并引物，利用RT-PCR和RACE技术首次从橡胶草（Taraxacum kok-saghyz）中克隆了一个HMGR基因，命名为TKHMGR。通过氨基酸序列同源性比对与系统进化分析表明，TKHMGR属于HMGR基因家族的新成员。同时，利用荧光定量方法分析了该基因在不同组织的表达情况。
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| 关 键 词： | 橡胶草；HMGR；基因克隆；序列分析；荧光定量 |
Cloning and Expression Analysis of HMGR Gene from Taraxacum kok-saghyz |
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| WANG Qi-CHao,LIU Shi-Zhong,XIAO Xian-Zhou. Cloning and Expression Analysis of HMGR Gene from Taraxacum kok-saghyz[J]. Bulletin of Botanical Research, 2012, 32(1): 61-68 |
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| Authors: | WANG Qi-CHao LIU Shi-Zhong XIAO Xian-Zhou |
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| Affiliation: | 1,2 (1.College of Horticulture and Landscape Architecture,Hainan University,Haikou 571737; 2.Rubber Research Institute,CATAS,Haikou 571737) |
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| Abstract: | Based on the design of degenerated oligonucleotides according to the conservative regions of nine 3-hydroxy-3-methylglutaryl-CoA reductases from nine plants and the total RNA extracted from Taraxacum kok-saghyz,a HMGR named TKHMGR was first obtained using the techniques of degenerate RT-PCR and RACE.Comparison of the amino acid sequence homology and system evolution analysis show that,TKHMGR belongs to the new members of the HMGR gene families.At the same time,the gene expression in different organizations was analyzed using fluorescence quantitative method.The cloning,bioinformatics analysis and expression analysis of this gene laid a foundation for further research on its function. |
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| Keywords: | Taraxacum kok-saghyz(TKS) 3-hydroxy-3-methyl-glutaryl-coenzyme A reductase(HMGR) gene cloning sequence analysis fluorescence quantitation |
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