粗茎鳞毛蕨孢子萌发研究

以经过3年低温储藏的粗茎鳞毛蕨孢子为实验材料,从孢子离心、孢子消毒、培养基种类、光质等4方面对孢子萌发进行研究,结果表明:在离心转数≤14 000 r.min-1、离心时间≤30 min条件下,离心处理对孢子萌发基本无影响;对孢子进行1%NaClO水溶液浸泡处理20～30 min为最佳消毒条件;改良Knop’s培养基为最佳孢子萌发培养基;黑暗条件下孢子不能萌发,但是黑暗处理能够明显提高孢子萌发整齐性;红光比白光能促进孢子提早萌发1 d左右,但对提高萌发率效果不显著。

Spore Germination of Dryopteris crassirhizoma (Dryopterydaceae)

In this paper,the spores of Dryopteris crassirhizoma after three years of low-temperature storage were used as the experimental material,the influences of centrifugal,disinfection,culture media and light quality on spore germination were respectively studied.The results showed that centrifugal(≤14000r·min-1,≤30min) almost had no influence on spore germination;Sterilizing spore surface for 10-20min with 1% NaClO was the best sterile method;Modified Knop’s medium was the best for spore germination;Spore could not germinate in dark,but the dark treatment could improve the regularity of spore germination;Red light could promote spore germinating about 1 day earlier than white light,but had no obvious influence on promoting spore germination rate.