蕤核叶片解剖结构和组织化学定位研究

采用解剖学和组织化学方法,研究了蕤核(Prinsepia uniflora Batal.)叶片的解剖结构,并对叶片中的黄酮类、生物碱类及多糖的组织化学定位进行了研究。结果表明:蕤核叶片上表皮有角质层,下表皮有气孔分布,气孔密度为278个·mm-2,近轴面栅栏组织细胞2～3层,排列紧密而整齐,含有许多晶体;叶片主脉木质部发达,由多列导管组成。上述特征说明蕤核叶片的解剖结构与环境之间的适应性。组织化学定位显示黄酮多分布于栅栏组织和厚角组织,生物碱含量少,多糖均匀分布于叶肉中。

Anatomical Structure and Histochemical Localization of the Leaf of Prinsepia uniflora Batal

The methods of anatomy and histochemistry were used to research the anatomical structure and histochemical localization of flavonoids,alkaloids and polysaccharides of the leaf of Prinsepia uniflora Batal.The results showed that the structure of the leaf of P.uniflora Batal was as follows: the upper epidermis cell with relative thick horny layer,the stomata existed in lower epidermis,the density of the stomata was 278mm-2.The upper side included 2 to 3 layers of palisade tissue cells,with a lot of crystals;the main vein of leaves had well developed xylem,with a number of radial arranged vessels.The characteristics mentioned above showed that P.uniflora Batal adapted well to the environment.The histochemical localization showed that: the total flavonoids mainly accumulated in the palisade tissue and collenchyma,alkaloids were poor existed in the leaf,polysaccharides were generally distributed in mesophyll of P.uniflora Batal.