Purification and properties of suppressor seryl-tRNA: ATP phosphotransferase from bovine liver |
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Authors: | T Mizutani A Hashimoto |
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Institution: | Faculty of Pharmaceutical Sciences, Nagoya City University, Mizuho-ku, Nagoya 467, Japan |
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Abstract: | Seryl-tRNASerCmCA: ATP phosphotransferase was purified 1200-fold from bovine liver by ultracentrifugation at 150 000 X g, chromatography on DEAE-cellulose, fractional precipitation with ammonium sulfate, chromatography on hydroxyapatite, gel filtration on Sephacryl S-300 and affinity chromatography on Blue Sepharose. Molecular mass was estimated as 135-145 kDa. The Km values for ATP and ser-tRNASerCmCA were 2 mM and 21 nM, respectively. This enzyme did not react with ser-tRNASerIGA, tyr-tRNA or thr-tRNA. |
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Keywords: | Bovine liver suppressor serine tRNA Phosphoseryl-tRNA Bovine liver phosphotransferase Serine tRNA Phosphotransferase purification Suppressor seryl-tRNA:ATP phosphotransferase |
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