Superoxide scavenging activity of pirfenidone-iron complex |
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Authors: | Mitani Yoshihiro Sato Keizo Muramoto Yosuke Karakawa Tomohiro Kitamado Masataka Iwanaga Tatsuya Nabeshima Tetsuji Maruyama Kumiko Nakagawa Kazuko Ishida Kazuhiko Sasamoto Kazumi |
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Institution: | a Division of Pharmacology & Therapeutics, Faculty of Medical and Pharmaceutical Sciences, Kumamoto University, 5-1 Oe-Honmachi, Kumamoto 862-0973, Japan b Dojindo Laboratories, Kumamoto, Japan c Dojin Glocal Corporation, Kumamoto, Japan |
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Abstract: | Pirfenidone (PFD) is focused on a new anti-fibrotic drug, which can minimize lung fibrosis etc. We evaluated the superoxide ( ) scavenging activities of PFD and the PFD-iron complex by electron spin resonance (ESR) spectroscopy, luminol-dependent chemiluminescence assay, and cytochrome c reduction assay. Firstly, we confirmed that the PFD-iron complex was formed by mixing iron chloride with threefold molar PFD, and the complex was stable in distillated water and ethanol. Secondary, the PFD-iron complex reduced the amount of produced by xanthine oxidase/hypoxanthine without inhibiting the enzyme activity. Thirdly, it also reduced the amount of released from phorbor ester-stimulated human neutrophils. PFD alone showed few such effects. These results suggest the possibility that the scavenging effect of the PFD-iron complex contributes to the anti-fibrotic action of PFD used for treating idiopathic pulmonary fibrosis. |
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Keywords: | 2 2-Dimethyl-3 4-dihydro-2H-pyrrole N-oxide (DMPO) Free radical Electron spin resonance Xanthine oxidase Neutrophil |
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