Gene expression patterns in glucose-stimulated podocytes |
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Authors: | Han Seung Hyeok Yang Sanghwa Jung Dong Sub Li Jin Ji Kim Jin Ju Kwak Seung Jae Kim Dong Ki Moon Sung Jin Lee Jung Eun Han Dae-Suk Kang Shin-Wook |
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Institution: | a Department of Internal Medicine, Yonsei University College of Medicine, 134 Shinchon-Dong Seodaemoon-Gu, Seoul 120-752, Republic of Korea b Brain Korea 21 Project for Medical Science, Yonsei University, Seoul, Republic of Korea c Cancer Metastasis Research Center, Yonsei University College of Medicine, Seoul, Republic of Korea d Department of Internal Medicine, Nephrology and Dialysis Unit, The Affiliated Hospital, YanBian University Medical College, JiLin, China |
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Abstract: | To explore the mechanisms of podocyte injury under diabetic conditions, we performed an expression profile in glucose-stimulated podocytes. Differential gene expression profiles between conditionally immortalized mouse podocytes cultured in medium containing 5.6 and 30 mM glucose were measured with oligonucleotide microarrays. Of the genes identified, heme oxygenase-1, vascular endothelial growth factor-A, and thrombospondin-1 showed a consistently increased pattern, whereas angiotensin-converting enzyme-2 and peroxisomal proliferator activator receptor-γ were down-regulated. These results were validated using real-time PCR and western blotting in podocytes, and with immunohistochemistry on renal tissues from streptozotocin-induced diabetic rats. Not only is this the first report of gene expression profiling of podocyte injury under diabetic conditions, but the identified genes are promising targets for future diabetes research. |
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Keywords: | Podocyte Gene expression Diabetic nephropathy |
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