Gluconate catabolism in cowpea rhizobia: evidence for a ketogluconate pathway |
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| Authors: | Mark D Stowers Gerald H Elkan |
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| Institution: | (1) NPI, University Research Park, 84108 Salt Lake City, UT, USA;(2) Department of Microbiology, North Carolina State University, P.O. Box 5476, 27650 Raleigh, NC, USA |
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| Abstract: | Enzymatic and radiorespirometric analysis of several strains of cowpea rhizobia revealed the presence of key enzymes of the Entner-Doudoroff (ED) pathway with the operation of the hexose cycle for the dissimilation of gluconate. These bacteria lack the oxidative pentose phosphate (PP) pathway when grown on gluconate. Gluconate-grown cells possessed an operational tricarboxylic acid (TAC) cycle. Enzymes of an ancillary pathway, the ketogluconate (KG) pathway for gluconate catabolism were detected. The presence of this pathway was confirmed by techniques of thin-layer chromatography and radiorespirometry.Abbreviations ED
Entner Doudoroff
- PP
pentose phosphate
- EMP
Embden-Meyerhof-Parnas
- KG
ketogluconate
- TCA
tricarboxylic acid
- DKG
diketogluconate
- PFK
phosphofructokinase |
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| Keywords: | Rhizobium-Rhizobium sp 32H1-cowpea rhizobia Gluconate catabolism Ketogluconate pathway |
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