Implication of tissue transglutaminase and desmoplakin in cell adhesion mechanism in human epidermis |
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| Authors: | Esposito Carla Lombardi Maria L. Ruocco Vincenzo Cozzolino Anna Mariniello Loredana Porta Raffaele |
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| Affiliation: | (1) Department of Biochemistry and Biophysics, 2nd University of Naples, via Costantinopoli 16, 80138 Naples, Italy;(2) Experimental Oncology - Immunology, National Institute of Cancer, 2nd University of Naples, via Costantinopoli 16, 80138 Naples, Italy;(3) Department of Dermatology, 2nd University of Naples, via Costantinopoli 16, 80138 Naples, Italy;(4) Department of Biochemistry and Biophysics, 2nd University of Naples, via Costantinopoli 16, 80138 Naples, Italy;(5) Department of Food Science, University of Naples, 'Federico II', Naples, Italy |
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| Abstract: | The distribution patterns of both tissue and keratinocyte transglutaminases (TGase), as well as that of desmoplakin (DP), have been immunohistochemically investigated in human skin cultured in the absence or presence of cystamine and enalapril, two acantholytic agents. In the control samples, tissue TGase is predominantly expressed in lower layers of the epidermis and is located intercellularly. Conversely, in tissues cultured with cystamine or enalapril, a diffuse cytoplasmatic staining was observed. Similarly, DP, detected on the cell membrane in the control, shifts into the cytosol of the keratinocytes following treatment. The distribution pattern of the keratinocyte enzyme in the acantholytic epidermis was identical to that observed in the normal one. Since cystamine and enalapril are TGase inhibitors and DP was shown to act as a TGase substrate in vitro, we suggest that DP and tissue enzyme may participate in cell adhesion at the intraepidermal level. |
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| Keywords: | acantholysis cystamine desmoplakin enalapril transglutaminase |
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