Phosphorylation of ornithine decarboxylase in intact erythroleukemia cells |
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| Authors: | F Flamigni S Marmiroli F Meggio C Guarnieri L A Pinna |
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| Institution: | Dipartimento di Biochimica, Università di Bologna, Italy. |
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| Abstract: | 32P-labeled ornithine decarboxylase was isolated by immunoprecipitation from murine erythroleukemia cells incubated in a medium containing 32P]ortophosphoric acid. Analysis of immunoprecipitate by SDS-polyacrylamide gel electrophoresis and autoradiography revealed a radiolabeled band, which corresponded to the position of mouse ornithine decarboxylase, phosphorylated in vitro by casein kinase-2. A preparation of casein kinase-2 purified from nuclei of erythroleukemia cells could also phosphorylate mouse ornithine decarboxylase. |
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