红树林湿地烷烃降解菌的分离筛选

从受石油污染的红树林湿地土样中分离筛选对烷烃具较高降解能力的细菌菌株, 以期应用于被石油污染滨海湿地的生物修复。采用富集培养方法, 富集、分离和筛选烷烃降解菌;观察各菌落及菌体形态特征;测试菌株Z2的生理生化特征, 并用16S rDNA序列分析方法进行该菌种鉴定。分离筛选得到Z1、Z2和Z3这3个能以正十六烷为唯一碳源生长的菌株, 其降解率依次为63.4%、82.5%和78.3%, 其中菌株Z2的降解率最高。经过形态学观察、生理生化特性实验和16S rDNA序列分析鉴定, 菌株Z2为不动杆菌(Acinetobacter sp.)。

Isolation and screening of hydrocarbon-degrading bacterial strains from mangrove wetland soils

Some hydrocarbon-degrading bacterial strains were isolated from the oil-contaminated mangrove soil on Qi-ao Island of Zhuhai City through selective enriched culture. There were three strains (named Z1, Z2 and Z3) being able to grow with hexadecane as sole carbon and energy resources. After 5 days culture, the degradation rates of Z1, Z2 and Z3 were 63.4%, 82.5% and 78.3%, respectively. The morphological and physiological-biochemical identification, 16S rDNA sequence analysis, and phylogenetic study showed that the strain Z2 belonged to genus Acinetobacter.