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Molecular cloning and nucleotide sequence of a cDNA clone coding for rat brain myelin proteolipid |
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| Authors: | A Dautigny P M Alliel L d'Auriol D Pham Dinh J L Nussbaum F Galibert P Jollès |
| Affiliation: | 1. Laboratoire des Protéines, Université de Paris V, 45, rue des Saints-Peres, 75270 Paris Cedex 06, France;2. Laboratoire d''Hématologie Expérimentale, Centre Hayem, Hôpital Saint-Louis, 2 place du Docteur Fournier, 75475 Paris Cedex 10, France;3. Institut de Neurochimie du CNRS, Laboratoire de Chimie Biologique, 67084 Strasbourg Cedex, France |
| Abstract: | A cDNA library from rat brain was constructed in pBR322 and screened with a 14-mer mixed oligonucleotide probe based on residues 231-235 of bovine proteolipid (PLP). A positive clone was isolated: it contained a 1334-base-pair cDNA insert and was subjected to DNA sequence analysis. The cDNA encoded information for the 276 amino acids of rat PLP. Comparison with bovine PLP sequence showed a complete amino acid sequence homology except for 4 amino acid residues. |
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