Cyclic nucleotide content of tobacco BY-2 cells |
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| Authors: | Richards Helen Das Swadipa Smith Christopher J Pereira Louisa Geisbrecht Alan Devitt Nicola J Games David E van Geyschem Jan Gareth Brenton A Newton Russell P |
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| Institution: | Biochemistry Group, Wallace Building, University of Wales Swansea, Singleton Park, Swansea SA2 8PP, UK. |
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| Abstract: | The cyclic nucleotide content of cultured tobacco bright yellow-2 (BY-2) cells was determined, after freeze-killing, perchlorate extraction and sequential chromatography, by radioimmunoassay. The identities of the putative cyclic nucleotides, adenosine 3',5'-cyclic monophosphate (cyclic AMP), guanosine 3',5'-cyclic monophosphate (cyclic GMP) and cytidine 3',5'-cyclic monophosphate (cyclic CMP) were unambiguously confirmed by tandem mass spectrometry. The potential of BY-2 cell cultures as a model system for future investigations of cyclic nucleotide function in higher plants is discussed. |
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| Keywords: | Tobacco BY2 Cyclic AMP Cyclic GMP Cyclic CMP Mass spectrometry Electrospray |
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