猪淋巴细胞表面E受体再生表达的免疫电镜观察

本文用酶标免疫电镜技术对猪外周淋巴细胞E受体在细胞表面的分布以及在细胞内合成代谢的状况进行了研究。实验结果表明:E受体在淋巴细胞表面呈点状均匀分布。胰蛋白酶处理使细胞表面E受体消失,经培养后可逐渐自发重建;正常情况下静止的淋巴细胞内E受体的合成水平很低;小牛胸腺肽制剂处理可以促进E受体在细胞质内的合成,加速其表达过程;更生霉素及嘌呤霉素等RNA合成抑制剂可抑制E受体的再生;以ConA活化的猪淋巴细胞内可见E受体活跃的合成;E受体合成部位是在粗面内质网上及散在的核蛋白体上。

OBSERVATIONS OF THE REGENERATION AND EXPRESSION OF E-RECEPTOR IN PORCINE LYMPHOCYTES BY IMMUNOELECTRON MICROSCOPY

Immunoelectron microscopy was used toobserve the distribution of E-receptor on thecell membrane and to localize its precursor inthe cytoplasm of porcine peripheral T lym-phocytes.E-receptor was shown to evenly dis-tribute on the lymphocyte surface.No reac-tion product could be found on the membraneof trypsinized lymphocytes.However,E-recep-tor could be re-established spontaneously withprolonged incubation of the cells.Inhibitorsof RNA synthesis such as puramycin and ac-tinomycin D blocked E-receptor regenerationwhereas calf thymic peptides induced its syn-thesis.The level of E-receptor synthesis inresting lymphocytes was too low to be detect-ed.Once the cells were activated by Con A.however,the synthesis was increased to suchan extent that the reaction product sticked tothe rough endoplasm reticulum could be seen,suggesting a certain correlation between E-receptor synthesis and the functional statusof T-cells.