Generation of a rabbit V(H) domain antibody polyspecific to c-Met and adenoviral knob protein |
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Authors: | Im Shin-Young Kim Ki Su Yun Chae-Ok Kim Joo-Hang Yi Kye-Sook Chung Junho |
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Institution: | Cancer Research Institute, Seoul National University College of Medicine, 28 Yongon-Dong, Chongno-gu, Seoul, Republic of Korea. |
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Abstract: | Several types of bispecific antibodies with affinity to both adenoviral coat proteins and a targeted antigen have been developed with the aim of providing the specific delivery of adenoviral gene therapy vehicle. From a phage display library of combinatorial dAb2s (each with an anti-adenoviral knob protein V(H) fragment linked with an anti-c-Met V(H)), we serendipitously enriched and isolated a clone, JS5, that has polyspecificity such that it binds both the adenoviral knob protein and c-Met, despite having only one V(H) domain. Our indirect observations suggest that the polyspecificity of JS5 is developed through accumulation of antibody specificity. The method of sequential immunization of a rabbit, first with the adenoviral knob protein and then with target antigens, may provide a method by which monoclonal antibodies with stand-alone polyspecificity may be developed. Such targeted polyspecific antibodies could readily be used for re-directing adenoviral vectors to target cells. |
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Keywords: | Bispecific antibody Domain antibody c-Met Adenoviral knob protein |
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