Transformation and expression of a staphylococcal plasmid in Escherichia coli |
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Authors: | Dilip Saha Manjusri Bal |
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Institution: | Department of Physiology, Section of Immunology and Microbiology, University College of Science and Technology, Calcutta, India |
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Abstract: | Abstract A multiple antibiotic-resistant Staphylococcus aureus , was found to possess three plasmid bands in agarose gel electrophoresis. A plasmid of approximately 4.3 kb (pMC790/2) was found to code for ampicillin and tetracycline resistance and to have one Eco RI site when transformed into S. aureus RN 4220. pMC790/2 in unmodified form was transformed into a recA? E. coli at a frequency of 1.2×104 transformants/μg of plasmid DNA. Plasmid (pMC790/2) replicated, maintained itself stably and expressed far better in the E. coli host than in S. aureus . |
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Keywords: | Plasmid S aureus Transformation Escherichia coli |
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