Analysis of the stability of cytochrome c(6) with an improved stopped-flow protocol |
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| Authors: | Lange Christian Hervás Manuel De la Rosa Miguel A |
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| Institution: | Instituto de Bioquímica Vegetal y Fotosíntesis, Centro de Investigaciones Científicas Isla de la Cartuja, Universidad de Sevilla y CSIC, Avda. Américo Vespucio s/n, 41092 Sevilla, Spain. |
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| Abstract: | This work presents an improved stopped-flow protocol for the simultaneous measurement of thermodynamic and kinetic protein stability data from a single experiment, along with a formalism for the global analysis of the data. The method was applied to the comparison of the stabilities of cytochrome c(6) from Anabaena sp. PCC 7119 and one of its mutants (D72K). Compared to the wild type the mutant was found to have a significantly reduced thermodynamic (deltadeltaG(U0)=2.7 kJ mol(-1)) and kinetic stability, as well as a more pronounced shift in transition state structure upon destabilization. |
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| Keywords: | Cytochrome c6 Kinetic analysis Protein folding Protein stability Stopped-flow Urea-induced denaturation |
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