Isolation of lipid activated pseudomurein precursors from Methanobacterium thermoautotrophicum |
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Authors: | Evamarie Hartmann Helmut König |
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Institution: | (1) Abteilung für Angewandte Mikrobiologie, Universität Ulm, Oberer Eselsberg M 23, D-7900 Ulm, Germany |
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Abstract: | From cell extracts of the pseudomurein possessing methanogen Methanobacterium thermoautotrophicum two putative pseudomurein precursors were isolated and characterized: (1) an undecaprenyl pyrophosphate activated disaccharide pentapeptide composed of N-acetylglucosamine, N-acetyltalosaminuronic acid, alanine, glutamic acid and lysine in a molar ratio of 1:1:2:2:1 and (2) the corresponding undecaprenyl pyrophosphate activated tetrapeptide lacking one alanine residue. The isolation of these precursors show that the biosynthesis of the eubacterial murein and the methano-bacterial pseudomurein differs not only in the cytoplasmic step, as recently described, but also in the lipid stage.Abbreviations GlcNitol
glucosaminitol
- NAcTalNUA
N-acetyltalosaminuronic acid
- Udp
undecaprenol
- TLC
thin layer chromatography |
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Keywords: | Methanogens Archaebacteria Methanobacterium thermoautotrophicum Cell walls Murein Pseudomurein Biosynthesis |
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