猪1号染色体微卫星多态性研究及遗传连锁图谱的构建

微卫星具有多态性高、保守性好等优点。本研究选取以20cM左右的间距均匀分布于1号染色体的8个多态微卫星基因座构建猪1号染色体的遗传连锁图谱，为进一步进行重要经济性状基因座的定位打下基础。试验结果表明，8个基因座等位基因数目2~5个，各个基因座等位基因频率在0.015~0.75之间，杂合度为0.39705~0.67675,多态信息含量为0.32925~0.59316。构建的资源家系遗传连锁图谱总长181.5cM,与USDA结果基本一致，可进一步用于猪数量性状基因座定位的研究。 The Microsatellite Polymorphism Research on Porcine Chromosome 1 and the Construction of Its Genetic MapQU Yan-chun,DENG Chang-yan,XIONG Yuan-zhu,SU Yu-hong,ZHENG Rong,LIU Gui-lanThe Key Laboratory of Pig Breeding and Genetics,The Ministry of Agriculture,Huazhong Agricultural University,Wuhan 430070,ChinaAbstract:As a molecular marker,microsatellite has many advantages such as high polymorphism and good conservativeness in animal genetic research.The study chose 8 microsatellite markers that evenly distributed on chromosome 1 with a distance about 20 cM to build the genetic map of porcine chromosome 1.The results of our experiment are as follows:the number of alleles for 8 markers is 2 to 5,their gene frequency is from 0.015 to 0.75,the heterozygosity is from 0.39705 to 0.67675 and the polymorphic information content is from 0.32925 to 0.59316.The map we built is basically in consistent with the result of USDA and can be used in searching quantitative traits loci in pigs.Key words：porcine; microsatellite; heterozygosity; polymorphic information content; genetic map

The Microsatellite Polymorphism Research on Porcine Chromosome 1 and the Construction of Its Genetic Map

As a molecular marker,microsatellite has many advantages such as high polymorphism and good conserva-tiveness in animal genetic research. The study chose 8 microsatellite markers that evenly distributed on chromosome 1 with a distance about 20 cM to build the genetic map of porcine chromosome 1. The results of our experiment are as follows:the number of alleles for 8 markers is 2 to 5,their gene frequency is from 0. 015 to 0. 75,the heterozygosi-ty is from 0. 39705 to 0. 67675 and the polymorphic information content is from 0. 32925 to 0. 59316. The map we built is basically in consistent with the result of USDA and can be used in searching quantitative traits loci in pigs.