Complete nucleotide sequence and molecular characterization of hemolysin II gene from Bacillus cereus |
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Authors: | Gleb Baidaa Zhanna I Budarinaa Nikolai P Kuzmina Alexander S Solonina |
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Affiliation: | Department of Molecular Microbiology, Centro de Investigaciones Biológicas, Consejo Superior de Investigaciones Cientı́ficas, Velázquez 144, 28006 Madrid, Spain |
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Abstract: | Hemolysin II gene from Bacillus cereus VKM-B771 has been sequenced. The deduced primary translation product consists of 412 amino acid residues which corresponds to the protein with an Mr of 45.6 kDa. The predicted mature Hly-II protein (residues 32 to 412) is of 42.3 kDa, which is in close agreement with the mini-cell electrophoresis analysis. Hly-II deletion variant lacking 96 C-terminal residues still has hemolytic activity. The protein primary structure analysis revealed no homology with any known Bacillus cytolysins. Significant general homology (31–28% identity) was found between the hemolysin II and Staphylococcus aureus alpha-toxin, gamma-hemolysin (HlgB), and leukocidins (LukF, LukF-R, LukF-PV). The data suggest that hemolysin II belongs to the group of β-channel forming cytolysins. |
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Keywords: | Phenylacetic acid Penicillin G acylase Pac regulation |
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