Enrichment of cardiac differentiation of mouse embryonic stem cells by optimizing the hanging drop method |
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Authors: | Chen Ming Lin Yong-Qing Xie Shuang-Lun Wu Hong-Fu Wang Jing-Feng |
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Institution: | (1) Department of Cardiology, Sun Yat-sen Memorial Hospital of Sun Yat-sen University, Guangzhou, People’s Republic of China;(2) Department of Physiology, Guangdong Medical College, Dongguan, People’s Republic of China; |
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Abstract: | Hanging drop (HD) culture is used to induce differentiation of embryonic stem cells (ESCs) into other cell types including
cardiomyocytes. However, the factors affecting cardiac differentiation of ESCs with this method remain incompletely understood.
We have investigated the effects of the starting number of ESCs in embryoid bodies (EBs) and the time of EB adherence to gelatin-coated
plates on cardiac differentiation: cardiac differentiation was increased in the EBs by a larger number of ESCs and was decreased
by plating EBs at day 4 or earlier. These two factors can thus be optimized to enrich the cardiac differentiation in ESCs
using the HD method. |
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