NAD+-glycohydrolase from Streptococcus pyogenes shows cyclic ADP-ribose forming activity |
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| Authors: | Tadahiro Karasawa Shin Takasawa Kiyotaka Yamakawa Hideto Yonekura Hiroshi Okamoto Shinichi Nakamura |
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| Institution: | Department of Bacteriology, School of Medicine, Kanazawa University, Kanazawa 920, Japan; Department of Biochemistry, Tohoku University School of Medicine, Sendai 980-77, Japan |
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| Abstract: | Abstract NAD+-glycohydrolase from Streptococcus pyogenes was purified by successive chromatography on CM Sepharose CL-6B, Sephacryl S-200 HR and hydroxyapatite. The purified enzyme possessed synthesis and hydrolysis activities of cyclic ADP-ribose (cADPR), a newly found second messenger for Ca2+ mobilisation, along with cleavage activity of the ribose-nicotinamide bond in NAD+. |
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| Keywords: | Cyclic ADP-ribose Streptococcus pyogenes NAD+-glycohydrolase ADP-ribosyl cyclase Cyclic ADP-ribose hydrolase |
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