Application of immunoproteomics to analysis of post-translational processing of the antiphagocytic M protein of Streptococcus |
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Authors: | Romer Terence G Boyle Michael D P |
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Affiliation: | Department of Microbiology and Immunology, Medical College of Ohio, Toledo, OH, USA. |
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Abstract: | Post-translational modification of the antiphagocytic M1 protein of Streptococcus pyogenes can influence its binding properties for human immunoglobulin G subclasses and its invasive potential. Current methods of monitoring this modification event involve N-terminal sequencing and are cumbersome, slow and not amenable to routine analysis. In this study we demonstrate that surface enhanced laser desorption/ionization-time of flight mass spectrometry can be used to monitor modification of the M1 protein by the secreted bacterial cysteine protease, SpeB. This method, when combined with a specific antibody capture step provides a specific, rapid and sensitive assay for key virulence factors of the important human pathogen Streptococcus pyogenes. |
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Keywords: | M protein Streptococcal pyogenic exotoxin B Streptococcus pyogenes Surface enhanced laser desorption/ionization |
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