Microsomal cytochromes of Candida tropicalis grown on alkanes |
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Authors: | Michel Gallo Bernadette Roche Edgard Azoulay |
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Affiliation: | Laboratoire de structure et function des biomembranes, E.R. 143, C.N.R.S., U.E.R. de Luminy, Département de Biologie, 13288, Marseille France |
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Abstract: | A comparison of methods used in isolating microsomes and in measuring microsomal cytochrome P-450 demonstrated that separation following protoplast lysis gave the best results. By this latter technique a high amount of cytochrome P-450 (0.2–0.3 nmol/mg) was recovered but cytochrome P-420, considered as the denatured form, was absent.The alkanes specifically induce cytochromes P-450 and b5 localized on the microsomes. The denaturation in vivo of cytochrome P-450 into cytochrome P-420 even occurs during storage at 1 °C. This degradation is increased during preparation of subcellular fractions if no preventive measures are taken. |
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