Immunoarchitecture of regenerated splenic transplants: Influence of donor and host age on the regeneration of splenic compartments |
| |
Authors: | Dr Jürgen Westermann Petra Peschel Reinhard Pabst |
| |
Institution: | (1) Center of Anatomy, Medical School of Hannover, Hannover, Germany;(2) Zentrum Anatomie 4150, Medizinische Hochschule Hannover, Postfach 610180, 61 Hannover, Germany |
| |
Abstract: | Summary Inbred rats were used as a model to determine the influence of the age of the implanted splenic tissue and the age of the host on the structure of transplanted splenic tissue. Monoclonal antibodies against lymphocyte, macrophage and dendritic cell subsets were used to evaluate the different compartments of the spleen. Adult rats received implants from adult, weanling or fetal rats, weanling rats received splenic tissue from adult, weanling or fetal rats and neonatal rats received neonatal or fetal spleens. There were major differences in the structure and cellular composition of the regenerated splenic tissue. The younger the recipients and the donor spleens, the better the normalization of the splenic compartments and the less fibrous tissue was found 3 months after transplantation. The follicles regenerated in all transplants, but the marginal zone was only normally developed in wealing and neonatal hosts. The periarteriolar lymphatic sheath regenerated in a similar manner to the marginal zone. Whenever a compartment developed, its cellular composition was the same as in a normal spleen. The immunhistological techniques enabled splenic regeneration to be characterized revealing a far from normal histological splenic structure in many age groups. These findings suggest that splenic regeneration in children might result in splenic tissue with normal compartments, which would be in contrast to some data in adults. |
| |
Keywords: | Spleen Splenic transplant Lymphocyte subsets Macrophage subsets Immunohistology Rat (Lewis) |
本文献已被 SpringerLink 等数据库收录! |
|