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Respective effects of glucose and glutamine on the glutamine synthetase activity of human skin fibroblasts |
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| Authors: | Théophile Soni Claire Wolfrom Samia Guerroui Nicole Raynaud Joséphine Poggi Nicole Moatti Marthe Gautier |
| Affiliation: | (1) Department of Cell Biology, Baylor College of Medicine, 77030 Houston, TX, USA;(2) Present address: Creighton Cancer Center, Creighton University, 24th at California Street, 68178 Omaha, NE;(3) Present address: Cancer and Developmental Biology, Syntex Research, R7-275, 94304 Palo Alto, CA, USA |
| Abstract: | Human A431 carcinoma cell line is known to have 30 fold amplified epidermal growth factor receptor (EGF-R) gene. We have studied the effect of steroid hormone dexamethasone (DEX) and protein synthesis inhibitor cycloheximide (CHX) on the expression of EGF-R gene in this cell line. DEX treatment and protein synthesis inhibition by CHX treatment cause a rapid 3 to 4 fold increase in the level of EGF-R mRNA and combined treatment of the above two agents have less than additive effect. It appears that mRNA for EGF-R accumulate within the cell during protein synthesis inhibition and upon removal of CHX, gets translated into EGF-R specific protein as judged by immuno-dot assay. We did not observe the phenomenon of  super induction nor much of an additive effect under condition of combined DEX and CHX treatment.Abbreviations EGF-R Epidermal Growth Factor Receptor - DEX Dexamethasone - CHX Cycloheximide |
| Keywords: | EGF-receptor gene gene expression cycloheximide dexamethasone and A431 carcinoma cell line |
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