二氧化硫体内衍生物对小鼠海马神经元DNA的损伤作用

运用单细胞凝胶电泳技术 (又称彗星实验 )研究了SO2 的体内代谢衍生物———亚硫酸氢钠(NaHSO3 )和亚硫酸钠 (Na2 SO3 )腹腔注射对小鼠海马神经元细胞DNA的损伤 .在衍生物剂量为 0 ,0 1 2 5、0 2 5 0、0 5 0 0、1 0 0 0g kg体重的染毒条件下 ,雄性小鼠海马神经元细胞DNA迁移长度分别为0 4 0、1 1 4、3 6 2、5 0 8、2 9 1 3μm ,雌性小鼠海马神经元细胞DNA迁移长度分别为 0 6 1 ,1 0 4 ,2 75 ,3 6 6 ,2 0 94 μm ,表明SO2 的体内衍生物可以引起小鼠海马神经元细胞DNA损伤 ,且随SO2 衍生物腹腔注射剂量的增加 ,DNA的损伤也加剧 .这意味着SO2 的体内衍生物具有引起哺乳动物海马神经元细胞DNA突变的潜在危险 ,表明SO2 衍生物是一种潜在的神经毒物

DNA Damage of Hippocampus Neurons in Mice by Sulfur Dioxide Derivatives

The damage effects of injected SO 2 derivatives intraperitoneally (ip) on freshly dissociated hippocampus neurons from mice were studied with the single cell microgel electrophoresis technique (Comet assay). After exposure to SO 2 derivatives at 0,0 125,0 250,0 500,1 000 g/kg body weight, tail lengths of nuclear DNA of hippocampus neurons from male mice were 0 40,1 14,3 62,5 08,29 13 μm, and female mice were 0 61,1 04,2 75,3 66,20 94 μm, respectively. It was showed that SO 2 derivatives could lead to DNA damage in hippocampus neurons. It means that SO 2 derivatives had a potential risk to cause DNA mutation of hippocampus neurons from mammalian animals, and it might have neurotoxic effects.