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Polyadenylic acid-containing RNA in Xenopus laevis oocytes
Authors:M Rosbash
Affiliation:Institute of Animal Genetics West Mains Road, Edinburgh EH9 3JN, Scotland;Department of Molecular Biology King''s Buildings, University of Edinburgh, Edinburgh EH9 3JN, Scotland
Abstract:The quantity of poly(A)-containing RNA is measured in Xenopus laevis oocytes as a function of developmental stage. The amount of poly(A)-containing RNA per oocyte, 0.7 to 1.0% of the total RNA, remains relatively constant from early vitellogenesis until ovulation. It is largely present in the cytoplasm of the oocyte in the form of a ribonucleoprotein complex. The poly(A) sequence is approximately 100 bases in length and is attached to molecules of heterogeneous sedimentation coefficients.
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