Genetic instability in EBV-transformed lymphoblastoid cell lines |
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Authors: | Mohyuddin Aisha Ayub Qasim Siddiqi Saima Carvalho-Silva Denise R Mazhar Kehkashan Rehman Sadia Firasat Sadaf Dar Ahsana Tyler-Smith Chris Mehdi S Qasim |
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Affiliation: | Biomedical and Genetic Engineering Division, Dr. A. Q. Khan Research Laboratories, G.P.O. Box 2891, Islamabad 44000, Pakistan. |
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Abstract: | Epstein Barr virus (EBV)-transformed lymphoblastoid cell lines are commonly used to provide an inexhaustible supply of DNA. We examined microsatellite instability in these cell lines in 35 individuals where DNA was available from the original blood samples and from cultured cell lines. Mutations were observed in 0.3% of the analyses, thus providing a quantitative measure of somatic mutation rate. |
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