Isolation and Characterization of Antifungal Substances from Burkholderia sp. Culture Broth |
| |
Authors: | Sopheareth Mao Seung-Je Lee Hoon Hwangbo Yong-Woong Kim Keun-Hyung Park Gyu-Suk Cha Ro-Dong Park Kil-Yong Kim |
| |
Affiliation: | (1) Department of Agricultural Chemistry, Division of Applied Bioscience and Biotechnology and Institute of Agricultural Science and Technology, Environmental-Friendly Agriculture Research Center, Chonnam National University, 300, Yong-Bong Dong, Buk-Ku, Gwangju, 500-757, South Korea;(2) Department of Food Science and Technology, Division of Applied Bioscience and Biotechnology and Institute of Agricultural Science and Technology, Chonman National University, Gwangju, 500-757, Korea;(3) Department of Environmental Engineering, Gwangju University, Gwangju, 503-703, Korea |
| |
Abstract: | A new antagonistic Burkholderia strain, designated MP-1 and producing antifungal activities against various filamentous plant pathogenic fungi, was isolated from the rhizoshere in the Naju area. Cultural characteristic studies strongly suggested that this strain belongs to the genus Burkholderia. The nucleotide sequence of the 16S rRNA gene (1491 pb) of strain MP-1 exhibited close similarity (99% to 100%) with other Burkholderia 16S rRNA genes. Extraction of fermentation broth of Burkholderia sp. MP-1 and various separations and purification steps led to isolation of four pure active molecules. The chemical structure of these four compounds—named phenylacetic acid, hydrocinnamic acid, 4-hydroxyphenylacetic acid, and 4-hydroxyphenylacetate methyl ester—was established on the basis on their gas chromatography–electron impact–mass spectrometry (GC-EI-MS) and trimethylsilation GC-EI-MS data. The four isolated compounds inhibited filamentous fungal growth on potato dextrose agar medium supplemented with 100 mg/L of phenylacetic acid, hydrocinnamic acid, 4-hydroxyphenylacetic acid and 4-hydroxyphenylacetate methyl ester individually. |
| |
Keywords: | |
本文献已被 PubMed SpringerLink 等数据库收录! |
|