Acetonitrile-based solvents: Their application in thin-layer chromatography of cyclic nucleotides, nucleosides, purine, and pyrimidines |
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| Authors: | Joseph F. Tomashefski Jr. Roberto J. Barrios Oscar Sudilovsky |
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| Affiliation: | Institute of Pathology, Case Western Reserve University and University Hospitals of Cleveland, Cleveland, Ohio 44106 USA |
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| Abstract: | Acetonitrile-based solvent mixtures have been applied to the separation of nucleotides, nucleosides, purines, pyrimidines, and cyclic nucleotides by thin-layer chromatography. The Rf's of over 35 compounds are presented. Development times with some of the systems were as low as 16 min. The use of acetonitrile-containing solvents in adenyl cyclase and cyclic phosphodiesterase assays and in the nucleotides and nucleic acid fields is discussed. |
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| Keywords: | To whom reprint requests should be made. |
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