Proteomic identification of differentially expressed proteins in the anoxic rice coleoptile |
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Authors: | Sadiq Irfan Fanucchi Francesca Paparelli Eleonora Alpi Emanuele Bachi Angela Alpi Amedeo Perata Pierdomenico |
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Affiliation: | a Plant Lab, Institute of Life Sciences, Scuola Superiore Sant’Anna, Pisa, Italy b Department of Crop Plant Biology, University of Pisa, Pisa, Italy c Biomolecular Mass Spectrometry Unit, San Raffaele Scientific Institute, Milan, Italy |
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Abstract: | Rice is the staple food for more than fifty percent of the world's population, and is therefore an important crop. However, its production is hindered by several biotic and abiotic stresses. Although rice is the only crop that can germinate even in the complete absence of oxygen (i.e. anoxia), flooding (low oxygen) is one of the major causes of reduced rice production. Rice germination under anoxia is characterized by the elongation of the coleoptile, but leaf growth is hampered. In this work, a comparative proteomic approach was used to detect and identify differentially expressed proteins in the anoxic rice coleoptile compared to the aerobic coleoptile. Thirty-one spots were successfully identified by MALDI-TOF MS analysis. The majority of the identified proteins were related to stress responses and redox metabolism. The expression levels of twenty-three proteins and their respective mRNAs were analyzed in a time course experiment. |
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Keywords: | 2-DE, two-dimensional gel electrophoresis ADF4, actin depolymerising factor 4 ADH, alcohol dehydrogenase ALDH, aldehyde dehydrogenase ANPs, anaerobic proteins APX, ascorbate peroxidise CBB, Comassie brilliant blue CBL, Calcineurin B-like protein CIPKs, CBL-interacting protein kinases GSTU, glutathione S-transferase IEF, isoelectric focusing IPG, immobilized pH gradient MS, mass spectrometry PDC, pyruvate decarboxylase ROS, reactive oxygen species sHSPs, small heat shock proteins SOD, superoxide dismutase TUBA1, tubulin α-1 chain Usp, universal stress protein |
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