Negative regulation of abscisic acid-induced stomatal closure by glutathione in Arabidopsis |
| |
Authors: | Eiji Okuma,Md. Sarwar Jahan,Shintaro Munemasa,Mohammad Anowar HossainDaichi Muroyama,Mohammad Mahbub IslamKen&rsquo ichi Ogawa,Megumi Watanabe-SugimotoYoshimasa Nakamura,Yasuaki ShimoishiIzumi C. Mori,Yoshiyuki Murata |
| |
Affiliation: | a Division of Bioscience, Graduate School of Natural Science and Technology, Okayama University, 1-1-1 Tsushima-Naka, Okayama 700-8530, Japan b Research Institute for Biological Sciences, Okayama, 7549-1 Yoshikawa, Kibichuo-cho, Okayama 716-1241, Japan c Institute of Plant Science and Resources, Okayama University, Okayama 710-0046, Japan |
| |
Abstract: | We found that glutathione (GSH) is involved in abscisic acid (ABA)-induced stomatal closure. Regulation of ABA signaling by GSH in guard cells was investigated using an Arabidopsis mutant, cad2-1, that is deficient in the first GSH biosynthesis enzyme, γ-glutamylcysteine synthetase, and a GSH-decreasing chemical, 1-chloro-2,4-dinitrobenzene (CDNB). Glutathione contents in guard cells decreased along with ABA-induced stomatal closure. Decreasing GSH by both the cad2-1 mutation and CDNB treatment enhanced ABA-induced stomatal closure. Glutathione monoethyl ester (GSHmee) restored the GSH level in cad2-1 guard cells and complemented the stomatal phenotype of the mutant. Depletion of GSH did not significantly increase ABA-induced production of reactive oxygen species in guard cells and GSH did not affect either activation of plasma membrane Ca2+-permeable channel currents by ABA or oscillation of the cytosolic free Ca2+ concentration induced by ABA. These results indicate that GSH negatively modulates a signal component other than ROS production and Ca2+ oscillation in ABA signal pathway of Arabidopsis guard cells. |
| |
Keywords: | ABA, abscisic acid CDNB, 1-chloro-2,4-dinitrobenzene [Ca2+]cyt, cytosolic free Ca2+ concentration GCP, guard cell protoplast GSB, glutathione S-bimane GSH, glutathione GSHmee, glutathione monoethyl ester GPX, glutathione peroxidase H2DCF-DA, 2&prime ,7&prime -dichlorofluorescin diacetate ICa, plasma membrane Ca2+-permeable channel MCB, monochlorobimane ROS, reactive oxygen species YC3.6, Yellow Cameleon 3.6 |
本文献已被 ScienceDirect 等数据库收录! |
|