Accession-dependent action potentials in Arabidopsis |
| |
Authors: | Favre Patrick Greppin Hubert Degli Agosti Robert |
| |
Affiliation: | a Laboratory of Plant Physiology and Biochemistry, Plant Physiomatics, University of Geneva, Switzerland b Plant Biology Department, University of Geneva, Switzerland c Plant Physiomatics, Plant Biology Department and Faculty of Sciences, University of Geneva, Switzerland d Plant Physiomatics, University of Applied Sciences Western Switzerland, Technology, Architecture and Landscape, Switzerland |
| |
Abstract: | Plant excitability, as measured by the appearance and circulation of action potentials (APs) after biotic and abiotic stress treatments, is a far lesser and more versatile phenomenon than in animals. To examine the genetic basis of plant excitability we used different Arabidopsis thaliana accessions. APs were induced by wounding (W) with a subsequent deposition (D) of 5 μL of 1 M KCl onto adult leaves. This treatment elicited transient voltage responses (APs) that were detected by 2 extracellular electrodes placed at a distance from the wounding location over an experimental time of 150 min. The first electrode (e1) was placed at the end of the petiole and the beginning of the leaf, and the second (e2) electrode was placed on the petiole near the center of the rosette. All accessions (Columbia (Col), Wassilewskija (Ws) and Landsberg erecta (Ler)) responded to the W & D treatment. After W & D treatment was performed on 100 plants for each accession, the number of APs ranged from 0 to 37 (median 8, total 940), 0 to 16 (median 5, total 528) and 0 to 18 (median 2, total 296) in Col, Ws and Ler, respectively. Responding plants (>0 APs) showed significantly different behaviors depending on their accessions of origin (i.e., Col 91, Ws 83 and Ler 76%). Some AP characteristics, such as amplitude and speed of propagation from e1 to e2 (1.28 mm s−1), were the same for all accessions, whereas the average duration of APs was similar in Col and Ws, but different in Ler. Self-sustained oscillations were observed more frequently in Col than Ws and least often in Ler, and the mean oscillation frequency was more rapid in Col, followed by Ws, and was slowest in Ler. In general, Col was the most excitable accession, followed by Ws, and Ler was the least excitable; this corresponded well with voltage elicited action potentials. In conclusion, part of Arabidopsis excitability in AP responses is genetically pre-determined. |
| |
Keywords: | AP, action potentials Col, Columbia Ler, Landsberg erecta Ws, Wassilewskija W & D, wound with a subsequent deposition of KCl |
本文献已被 ScienceDirect PubMed 等数据库收录! |
|