Elucidation of structure-function relationships in photosynthetic light-harvesting antenna complexes by non-linear polarization spectroscopy in the frequency domain (NLPF) |
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Authors: | Lokstein Heiko Krikunova Maria Teuchner Klaus Voigt Bernd |
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Institution: | a Institut für Biochemie und Biologie/Pflanzenphysiologie, Universität Potsdam, Karl-Liebknecht-Str. 24-25, Haus 20, D-14476 Potsdam-Golm, Germany b Institut für Physik und Astronomie/Photonik, Universität Potsdam, Haus 28, D-14476 Potsdam-Golm, Germany c Technische Universität Berlin, Institut für Optik und Atomare Physik, Hardenbergstrasse 36, D-10623 Berlin, Germany |
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Abstract: | Photosynthetically active pigments are usually organized into pigment-protein complexes. These include light-harvesting antenna complexes (LHCs) and reaction centers. Site energies of the bound pigments are determined by interactions with their environment, i.e., by pigment-protein as well as pigment-pigment interactions. Thus, resolution of spectral substructures of the pigment-protein complexes may provide valuable insight into structure-function relationships. By means of conventional (linear) and time-resolved spectroscopic techniques, however, it is often difficult to resolve the spectral substructures of complex pigment-protein assemblies. Nonlinear polarization spectroscopy in the frequency domain (NLPF) is shown to be a valuable technique in this regard. Based on initial experimental work with purple bacterial antenna complexes as well as model systems NLPF has been extended to analyse the substructure(s) of very complex spectra, including analyses of interactions between chlorophylls and "optically dark" states of carotenoids in LHCs. The paper reviews previous work and outlines perspectives regarding the application of NLPF spectroscopy to disentangle structure-function relationships in pigment-protein complexes. |
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Keywords: | Chl chlorophyll CP29 minor chlorophyll-binding protein of photosystem II CP47 core antenna chlorophyll-protein of photosystem II C-PC cyanobacterial phycocyanin LHC II light-harvesting complex II NLPF nonlinear polarization spectroscopy in the frequency domain PCP peridinin-chlorophyll-a-protein EET excitation energy transfer RT room temperature |
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