Nitric oxide content is associated with tolerance to bicarbonate-induced chlorosis in micropropagated Prunus explants |
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Authors: | Cellini Antonio Corpas Francisco Javier Barroso Juan Bautista Masia Andrea |
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Affiliation: | a Dipartimento di Colture Arboree, Università degli Studi di Bologna, Viale Fanin 46, 40127 Bologna, Italy b Departamento de Bioquímica, Biología Celular y Molecular de Plantas, Estación Experimental del Zaidín (EEZ), CSIC, E-18080 Granada, Spain c CSIC (EEZ) Associated Unit, Universidad de Jaén, Biochemistry and Molecular Biology, Campus Las Lagunillas, Jaen E-23071, Spain |
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Abstract: | Iron (Fe) chlorosis is a common nutritional deficiency in fruit trees grown in calcareous soils. Grafting on tolerant rootstocks is the most efficient practice to cope with it. In the present work, three Prunus hybrid genotypes, commonly used as peach rootstocks, and one peach cultivar were cultivated with bicarbonate in the growth medium. Parameters describing oxidative stress and the metabolism of reactive nitrogen species were studied. Lower contents of nitric oxide and a decreased nitrosoglutathione reductase activity were found in the most sensitive genotypes, characterized by higher oxidative stress and reduced antioxidant defense. In the peach cultivar, which behaved as a tolerant genotype, a specifically nitrated polypeptide was found. |
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Keywords: | APX, ascorbate-dependent peroxidase CAPS, 3-(cyclohexylamino)-1-propanesulfonic acid CAT, catalase DAF-2, 4,5-diaminofluorescein DTT, dithiotreitol EDTA, ethylenediaminetetraacetic acid FCR, Fe(III)-chelate reductase GPX, guaiacol-dependent peroxidase GSNO, nitrosoglutathione GSNOR, nitrosoglutathione reductase GSSG, oxidized glutathione HEPES, 4-(2-hydroxyethyl)piperazine-1-ethanesulfonic acid MDA, malondialdehyde NADH, β-nicotinamide adenine dinucleotide reduced form NADPH, β-nicotinamide adenine dinucleotide phosphate reduced form NBT, nitro blu tetrazolium NO, nitric oxide PVPP, polyvinylpolypyrrolidone RNS, reactive nitrogen species ROS, reactive oxygen species SDS-PAGE, Na dodecyl sulfate-polyacrilamide gel electrophoresis SOD, superoxide dismutase TEMED, N,N,N&prime ,N&prime -tetramethylethylenediamine TRIS, tris(hydroxymethyl)aminomethane |
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