Inoculation with Xanthomonas oryzae pv. oryzae induces thylakoid membrane association of Rubisco activase in Oryza meyeriana |
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| Authors: | Yang Yong Yu Chu-Lang Wang Xu-Ming Yan Cheng-Qi Cheng Ye Chen Jian-Ping |
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| Affiliation: | MOA and Zhejiang Provincial Key Laboratory of Plant Virology, Institute of Virology and Biotechnology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China |
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| Abstract: | Oryza meyeriana is a wild species of rice with high resistance to Xanthomonas oryzae pv. oryzae (Xoo), but the resistance mechanism is poorly understood. Protein gel blot analysis and immuno-gold electron microscopy showed that Xoo infection induced an association of ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) activase (RCA) with the thylakoid membrane in O. meyeriana, which led to considerable decline in the initial activity and the activation state of Rubisco. In susceptible cultivated rice, RCA remained in the chloroplast stroma. RCA may play a role in resistance to Xoo in O. meyeriana that differs from its well-known role in activating Rubisco, perhaps by protecting the thylakoid membrane against damage from Xoo. |
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| Keywords: | Rubisco, ribulose-1,5-bisphosphate carboxylase/oxygenase RCA, Rubisco activase ROS, reactive oxygen species Xoo, Xanthomonas oryzae pv. oryzae PVDF, polyvinylidene fluoride NBT, nitroblue tetrazolium BCIP, 5-bromo-4-chloro-3-indolyl phosphate |
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